Broadly neutralizing anti-HIV monoclonal antibody PGT 135

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody PGT 135
Associated Publication	Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120. Kong L, Lee JH, Doores KJ, Murin CD, Julien JP, McBride R, Liu Y, Marozsan A, Cupo A, Klasse PJ, Hoffenberg S, Caulfield M, King CR, Hua Y, Le KM, Khayat R, Deller MC, Clayton T, Tien H, Feizi T, Sanders RW, Paulson JC, Moore JP, Stanfield RL, Burton DR, Ward AB, Wilson IA Nature structural & molecular biology, 20, 796-803 2013 Jul
Analyte Name	PGT 135
Analyte Family	Antibodies
Analyte Information	One of the human HIV-1 broad and potent neutralizing IgG antibodies isolated from HIV-1-infected individuals by single-cell sorting and neutralization screen and appears to bind to an Asn332 glycan-dependent epitope on gp120.
Concentration	10 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), PGT 135 was precomplexed with biotinylated anti-human IgG (Vector) at a 1:3 ratio (w/w) before application onto the slides at a final concentration of 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml).
Other Comments	N/A