Broadly neutralizing anti-HIV monoclonal antibody 2G12_2010 data with 47 N-glycan-related probes

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody 2G12_2010 data with 47 N-glycan-related probes
Associated Publication	A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA Science (New York, N.Y.), 334, 1097-103 2011 Nov 25
Analyte Name	2G12
Analyte Family	Antibodies
Analyte Information	Broadly neutralizing human monoclonal IgG antibody against HIV-1 that has an epitope centred on the high-mannose and/or hybrid N-glycan residues on the viral spike glycoprotein gp120.
Concentration	1/10000
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), human 2G12 was assayed at 1/10000, followed by biotinylated anti-human IgG (Vector) 1:200. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	Note: The data were from the same microarray screening experiments as in Dunlop et al Glycobiology 2010 paper, but with a different probe filter.