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Concanavalin A (ConA)

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Instructions | Symbol Definitions | Lipid Tag Descriptions
(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

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Experiment NameConcanavalin A (ConA)
Associated PublicationA potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield.
Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA
Science (New York, N.Y.), 334, 1097-103
2011 Nov 25
Analyte NameConA
Analyte FamilyPlant lectins
Analyte InformationBiotinylated Concanavalin A (ConA) from VECTOR (B-1005).
Concentration0.5 ug/mL
ProtocolAfter blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), biotinylated ConA was assayed at 0.5 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other CommentsN/A