Broadly neutralizing anti-HIV monoclonal antibody PGT 127

Instructions | Symbol Definitions | Lipid Tag Descriptions
(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

Sort By Value
Display Errors

Textual Symbolic

Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody PGT 127
Associated Publication	A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA Science (New York, N.Y.), 334, 1097-103 2011 Nov 25
Analyte Name	PGT 127
Analyte Family	Antibodies
Analyte Information	One of the human HIV-1 broadly and potent neutralizing IgG antibodies isolated from HIV-1-infected individuals by single-cell sorting and neutralization screen and appears to bind to an Asn332 glycan-dependent epitope on gp120.
Concentration	10 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), PGT 127 was precomplexed with biotinylated anti-human IgG (Vector) at a 1:3 ratio (w/w) before application onto the slides at a final concentration of 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml).
Other Comments	N/A