(His)6-MBP tag (control)

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Experiment Name	(His)6-MBP tag (control)
Associated Publication	Structural basis for multiple sugar recognition of Jacalin-related human ZG16p lectin. Kanagawa M, Liu Y, Hanashima S, Ikeda A, Chai W, Nakano Y, Kojima-Aikawa K, Feizi T, Yamaguchi Y The Journal of biological chemistry, 289, 16954-65 2014 Jun 13
Analyte Name	MBP-His
Analyte Family	Other microbial proteins
Analyte Information	Hexa-His-tagged maltose-binding protein (MBP) was expressed in Escherichia coli as a control for (His)6-MBP-fused ZG16p
Concentration	200 ug/ml
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in PBS, the (His)6-MBP protein was overlaid onto the arrays at 200 ug/ml (undiluted) followed and anti-His and biotinylated precompleced with anti-mouse IgG (both from Sigma and used at 10 ug/ml). Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml).
Other Comments	N/A