anti-blood group B antibody

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Experiment Name	anti-blood group B antibody
Associated Publication	Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array. Gao C, Liu Y, Zhang H, Zhang Y, Fukuda MN, Palma AS, Kozak RP, Childs RA, Nonaka M, Li Z, Siegel DL, Hanfland P, Peehl DM, Chai W, Greene MI, Feizi T The Journal of biological chemistry, 289, 16462-77 2014 Jun 6
Analyte Name	Monoclonal antibody Anti-B (89-F)
Analyte Family	Antibodies
Analyte Information	Monoclonal antibody Anti-B (89-F) was from Santa Cruz.
Concentration	1 ug/ml
Protocol	After blocking with 3% w/v BSA in HEPES-buffered saline (5 mM HEPES, pH 7.4, 150mM NaCl, 5 mM CaCl2), anti-B was overlaid both at 1 ug/ml at 20 C. Bnding was detected using biotinylated anti-mouse IgG (Sigma) at 1:200 dilution followed by Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA