Reassortant influenza H3N2 A/Aichi/2/68 (X31)_1:10

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Experiment Name	Reassortant influenza H3N2 A/Aichi/2/68 (X31)_1:10
Associated Publication	Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray. Childs RA, Palma AS, Wharton S, Matrosovich T, Liu Y, Chai W, Campanero-Rhodes MA, Zhang Y, Eickmann M, Kiso M, Hay A, Matrosovich M, Feizi T Nature biotechnology, 27, 797-9 2009 Sep
Analyte Name	X31 A/Aichi/2/68, H3N2 virus
Analyte Family	Viruses/Virus-like particles
Analyte Information	Beta-propiolactone inactivated reassortant human H3N2 virus, grown in 10 day old embryonated eggs.
Concentration	10 ug HA/ml
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577), 0.02% Blocker Casein (Pierce) in 150mM sodium chloride and 5 mM calcium chloride, the suspension of the inactivated virus (10 ug/ml) was assayed in the presence of 17.5 uM oseltamivir carboxylate. After fixation with 4% paraformaldehyde, bound viruses were detected using the rabbit antiserum to A/Aichi/2/68 (X31) (1:200), followed by biotinylated Protein A (Sigma), 10 ug/ml, and Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	In this publication suspensions of the viruses were adjusted to contain equivalent concentrations of HA glycoprotein as assessed by gel electrophoresis; we refer to these as standard concentrations. This experiment was at 1:10 dilution of virus standard c