Peanut agglutinin (PNA)

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Experiment Name	Peanut agglutinin (PNA)
Associated Publication	The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins. Palma AS, Liu Y, Childs RA, Herbert C, Wang D, Chai W, Feizi T Biochemical and biophysical research communications, 408, 548-52 2011 May 20
Analyte Name	PNA
Analyte Family	Plant lectins
Analyte Information	Biotinylated Peanut Agglutinin (PNA) from VECTOR (B1075).
Concentration	5 ug/ml
Protocol	After blocking arrayed slides with 3% w/v bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), biotinylated PNA was assayed at 5 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA