Leukocyte receptor Dectin-1 (murine)_His tagged_2016 data

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(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

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Experiment Name	Leukocyte receptor Dectin-1 (murine)_His tagged_2016 data
Associated Publication	Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system. Zhang H, Palma AS, Zhang Y, Childs RA, Liu Y, Mitchell DA, Guidolin LS, Weigel W, Mulloy B, Ciocchini AE, Feizi T, Chai W Glycobiology, 26, 1086-1096 2016 Oct
Analyte Name	Murine His-Dectin-1
Analyte Family	Animal lectins
Analyte Information	Extracellular domain (Phe 69-Leu 244) of murine Dectin-1 fused with an N-terminal hexa-histidine tag and recombinantly expressed in human cells (50233-M07H; Sino Biological, China). Dectin-1 is a signalling C-type-lectin-like receptor for fungal Beta-glucans on immune cells mediating innate immunity to fungal pathogens.
Concentration	20 ug/ml
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), murine His-Dectin-1 was precomplexed with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) in a ratio of 1:3:3 (by weight) and overlaid onto the arrays at 20 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	The results of 20 selected oligosaccharide probes at 2 and 5 fmol per spot are shown. The full microarray was published in Palma et al 2015 Molecular & cellular proteomics: MCP, 14, 974-88