DC-SIGN closely related human receptor DC-SIGNR (or L-SIGN)_Biotinylated

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Experiment Name	DC-SIGN closely related human receptor DC-SIGNR (or L-SIGN)_Biotinylated
Associated Publication	Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system. Zhang H, Palma AS, Zhang Y, Childs RA, Liu Y, Mitchell DA, Guidolin LS, Weigel W, Mulloy B, Ciocchini AE, Feizi T, Chai W Glycobiology, 26, 1086-1096 2016 Oct
Analyte Name	DC-SIGNR (L-SIGN)-biotin
Analyte Family	Animal lectins
Analyte Information	Recombinant DC-SIGNR (complete extracellular domain lacking the transmembrane domain) was recombinantly expressed in Escherichia coli (Mitchell et al. 2001, J Biol Chem 276: 28939-28945) and analysed in the microarrays in a biotinylated form.
Concentration	50 ug/ml
Protocol	After blocking arrayed slides with 0.02% Blocker Casein (Pierce) containing 1% (w/v) bovine serum albumin (Sigma A8577) and 5 mM CaCl2 in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl), DC-SIGNR-biotin was analysed at 50 μg/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	The results of 20 selected oligosaccharide probes at 2 and 5 fmol per spot are shown. The full microarray was published in Palma et al 2015 Molecular & cellular proteomics: MCP, 14, 974-88