Human adenoviruse type 52 short fiber knob (HAdV52 SFK)

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(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

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Experiment Name	Human adenoviruse type 52 short fiber knob (HAdV52 SFK)
Associated Publication	Human adenovirus 52 uses sialic acid-containing glycoproteins and the coxsackie and adenovirus receptor for binding to target cells. Lenman A, Liaci AM, Liu Y, Årdahl C, Rajan A, Nilsson E, Bradford W, Kaeshammer L, Jones MS, Frängsmyr L, Feizi T, Stehle T, Arnberg N PLoS pathogens, 11, e1004657 2015 Feb
Analyte Name	HAdV52 SFK
Analyte Family	Viral proteins
Analyte Information	DNA fragment encoding the knob domain of the short fiber (SFK) was isolated from virions of Species G HAdV-52 (strain TB3-2243) and cloned into a pQE30Xa expression vector encoding an N-terminal His-tag. The protein HAdV52 SFK was expressed in Escherichia coli (strain M15) and purified with Ni-NTA agarose beads.
Concentration	150 ug/ml
Protocol	After blocking arrayed slides with 0.3% (v/v) Blocker Casein (Pierce), 0.3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), HAdV52 SFK was precomplexed with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) in a ratio of 4:2:1 (by weight) and overlaid onto the arrays at 150 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	The results of 15 oligosaccharide probes at 5 fmol per spot are shown. The full microarray data will be described elsewhere.