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Concanavalin A (ConA)

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Instructions | Symbol Definitions | Lipid Tag Descriptions
(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

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Experiment NameConcanavalin A (ConA)
Associated PublicationUnravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry.
Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W
Molecular & cellular proteomics : MCP, 14, 974-88
2015 Apr
Analyte NameConA
Analyte FamilyPlant lectins
Analyte InformationBiotinylated Concanavalin A (ConA) from VECTOR (B-1005).
Concentration2 ug/ml
ProtocolAfter blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), biotinylated ConA was assayed at 2 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other CommentsN/A