Anti-betα-glucan antibody mAb 1H8-IgG

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Experiment Name	Anti-betα-glucan antibody mAb 1H8-IgG
Associated Publication	Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W Molecular & cellular proteomics : MCP, 14, 974-88 2015 Apr
Analyte Name	mAb 1H8-IgG
Analyte Family	Antibodies
Analyte Information	Murine hybridoma IgG antibody, produced by Balb/c mouse immunization with a glyco-conjugate vaccine containing a soluble Candida albicans beta-glucan preparation (GGZym) conjugated to the recombinant, genetically inactivated diphtheria toxin CRM197. This antibody is potentially protective in fungal infection.
Concentration	0.1 ug/ml
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), mAb 1H8-IgG was assayed at 0.1 ug/ml, followed by biotinylated anti-mouse IgG (Sigma) at 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A