Anti-dextran 16.412E-IgA mAb (Cavity-type)

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Experiment Name	Anti-dextran 16.412E-IgA mAb (Cavity-type)
Associated Publication	Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W Molecular & cellular proteomics : MCP, 14, 974-88 2015 Apr
Analyte Name	Anti-dextran m16.4.12E-IgA
Analyte Family	Antibodies
Analyte Information	Monoclonal cavity-type anti-dextran antibody, from the Elvin Kabat collection, produced after immunization of mice with isomaltosyl hexasaccharide- keyhole limpet hemocyanin, purified and biotinylated.
Concentration	1/500
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), biotinylated anti-dextran m16.4.12E-IgA was assayed at 1/500. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A