Dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN)_Fc tagged

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Experiment Name	Dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN)_Fc tagged
Associated Publication	Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W Molecular & cellular proteomics : MCP, 14, 974-88 2015 Apr
Analyte Name	Fc-DC-SIGN
Analyte Family	Animal lectins
Analyte Information	Extracellular domain of DC-SIGN (amino acid residues 64-404) fused at the C terminus to a human IgG1-Fc fragment and recombinantly expressed in CHO cells (DC-SIGN-Fc). DC-SIGN is a signalling C-type-lectin receptor of the immune system that plays a role in cell-cell and host-pathogen interactions.
Concentration	2 ug/ml
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), Fc-DC-SIGN was assayed at 2 ug/ml, followed by biotinylated anti-human IgG (Vector) at 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A