Leukocyte receptor Dectin-1 (murine)_His tagged_2015 data

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Experiment Name	Leukocyte receptor Dectin-1 (murine)_His tagged_2015 data
Associated Publication	Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W Molecular & cellular proteomics : MCP, 14, 974-88 2015 Apr
Analyte Name	Murine His-Dectin-1
Analyte Family	Animal lectins
Analyte Information	Extracellular domain (Phe 69-Leu 244) of murine Dectin-1 fused with an N-terminal hexa-histidine tag and recombinantly expressed in human cells (50233-M07H; Sino Biological, China). Dectin-1 is a signalling C-type-lectin-like receptor for fungal Beta-glucans on immune cells mediating innate immunity to fungal pathogens.
Concentration	15 ug/ml
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), murine His-Dectin-1 was precomplexed with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) in a ratio of 1:3:3 (by weight) and overlaid onto the arrays at 15 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A