Rabbit serum immunized with wild-type S. cerevisiae (WT)

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Experiment Name	Rabbit serum immunized with wild-type S. cerevisiae (WT)
Associated Publication	Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans. Dunlop DC, Bonomelli C, Mansab F, Vasiljevic S, Doores KJ, Wormald MR, Palma AS, Feizi T, Harvey DJ, Dwek RA, Crispin M, Scanlan CN Glycobiology, 20, 812-23 2010 Jul
Analyte Name	S. cerevisiae WT immune serum
Analyte Family	Antibodies
Analyte Information	Antiserum elicited after rabbit immunization with wild-type Saccharomyces cerevisiae.
Concentration	1/25
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), S. cerevisiae WT immune serum was assayed at 1/25, followed by biotinylated anti-rabbit IgG (Vector) 1:200. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A