Broadly neutralizing anti-HIV monoclonal antibody 2G12_2010 data

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody 2G12_2010 data
Associated Publication	Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans. Dunlop DC, Bonomelli C, Mansab F, Vasiljevic S, Doores KJ, Wormald MR, Palma AS, Feizi T, Harvey DJ, Dwek RA, Crispin M, Scanlan CN Glycobiology, 20, 812-23 2010 Jul
Analyte Name	2G12
Analyte Family	Antibodies
Analyte Information	Broadly neutralizing human monoclonal IgG antibody against HIV-1 that binds a N-glycan epitope on the viral spike glycoprotein, gp120.
Concentration	1/10000
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), human 2G12 was assayed at 1/10000, followed by biotinylated anti-human IgG (Vector) 1:200. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A