Leukocyte receptor CLECSF8 (murine)_Fc tagged

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Experiment Name	Leukocyte receptor CLECSF8 (murine)_Fc tagged
Associated Publication	The C-type lectin receptor CLECSF8 (CLEC4D) is expressed by myeloid cells and triggers cellular activation through Syk kinase. Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD The Journal of biological chemistry, 287, 25964-74 2012 Jul 27
Analyte Name	Murine Fc-CLECSF8
Analyte Family	Animal lectins
Analyte Information	Extracellular domain of murine CLECSF8 fused to Fc portion of human IgG1 and recombinantly expressed in HEL293T cells (Murine CLECSF8 human IgG Fc chimera, Fc-CLECSF8). CLECSF8 is a signalling receptor member of the 'Dectin-2 cluster' of C-type-lectin receptors and a key molecule in anti-mycobacterial host defence.
Concentration	20 ug/ml
Protocol	After blocking arrayed slides with 1% (w/v) bovine serum albumin (Sigma A8577) in Pierce Blocker solution (casein/BSA), Fc-CLECSF8 was assayed at 20 ug/ml followed by biotinylated anti-human IgG at 3 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A