Anti-betα-glucan antibody mAb 1E12-IgM_2009 data

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Experiment Name	Anti-betα-glucan antibody mAb 1E12-IgM_2009 data
Associated Publication	Protection by anti-beta-glucan antibodies is associated with restricted beta-1,3 glucan binding specificity and inhibition of fungal growth and adherence. Torosantucci A, Chiani P, Bromuro C, De Bernardis F, Palma AS, Liu Y, Mignogna G, Maras B, Colone M, Stringaro A, Zamboni S, Feizi T, Cassone A PloS one, 4, e5392 2009
Analyte Name	mAb 1E12-IgM
Analyte Family	Antibodies
Analyte Information	Murine hybridoma IgM antibody, produced by Balb/c mouse immunization with a glyco-conjugate vaccine containing a soluble Candida albicans beta-glucan preparation (GGZym) conjugated to the recombinant, genetically inactivated diphteria toxin CRM197. mAb 1E12-IgM was shown to bind to the cell wall of fungal pathogens (Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans) but did not confer protection in an experimental model of C. albicans infection.
Concentration	1/1000
Protocol	After blocking arrayed slides with 1% (w/v) bovine serum albumin (Sigma A8577) in Pierce Blocker solution (casein/BSA) containing 10 mM CaCl2, mAb 1E12-IgM was assayed at 0.5 ug/ml, followed by biotinylated anti-mouse Igs (Sigma) 1:1000. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A