Broadly neutralizing anti-HIV anti-V3 loop antibody 10-188

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Experiment Name	Broadly neutralizing anti-HIV anti-V3 loop antibody 10-188
Associated Publication	Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies. Mouquet H, Scharf L, Euler Z, Liu Y, Eden C, Scheid JF, Halper-Stromberg A, Gnanapragasam PN, Spencer DI, Seaman MS, Schuitemaker H, Feizi T, Nussenzweig MC, Bjorkman PJ Proceedings of the National Academy of Sciences of the United States of America, 109, E3268-77 2012 Nov 20
Analyte Name	10-188
Analyte Family	Antibodies
Analyte Information	An anti-V3 loop antibody (Mouquet H, et al. PLoS One 6(9):e24078. 2011) used as a positive control antibody in the studies of binding activities of antibodies in the PGT121-like and 10-1074-like groups.
Concentration	50 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), 10-188 was analyzed at 50 ug/ml, followed by biotinylated anti-human IgG (Vector) 5 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A