The 10-1074 'glycomutant' antibody, 10-1074GM_2012 data

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Experiment Name	The 10-1074 'glycomutant' antibody, 10-1074GM_2012 data
Associated Publication	Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies. Mouquet H, Scharf L, Euler Z, Liu Y, Eden C, Scheid JF, Halper-Stromberg A, Gnanapragasam PN, Spencer DI, Seaman MS, Schuitemaker H, Feizi T, Nussenzweig MC, Bjorkman PJ Proceedings of the National Academy of Sciences of the United States of America, 109, E3268-77 2012 Nov 20
Analyte Name	10-1074GM
Analyte Family	Antibodies
Analyte Information	The mutant antibody of 10-1074 designed to exchange the complex-type glycan-contacting residues between PGT121 and 10-1074. It contains six mutation sites (Y32S, D53K, R54S, T58N, R97H, and Y100lT) that are crystallographically identified glycan contact residues..
Concentration	50 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), P10-1074GM was analyzed at 50 ug/ml, followed by biotinylated anti-human IgG (Vector) 5 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A