Broadly neutralizing anti-HIV monoclonal antibody PGT 121_2014 data

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody PGT 121_2014 data
Associated Publication	Broadly neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope trimers. Falkowska E, Le KM, Ramos A, Doores KJ, Lee JH, Blattner C, Ramirez A, Derking R, van Gils MJ, Liang CH, Mcbride R, von Bredow B, Shivatare SS, Wu CY, Chan-Hui PY, Liu Y, Feizi T, Zwick MB, Koff WC, Seaman MS, Swiderek K, Moore JP, Evans D, Paulson JC, Wong CH, Ward AB, Wilson IA, Sanders RW, Poignard P, Burton DR Immunity, 40, 657-68 2014 May 15
Analyte Name	PGT 121
Analyte Family	Antibodies
Analyte Information	One of the human HIV-1 broad and potent neutralizing IgG antibodies isolated from HIV-1-infected individuals by single-cell sorting and neutralization screen and appears to bind to an Asn332 glycan-dependent epitope on gp120.
Concentration	50 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), PGT121 was analyzed at 50 ug/ml, followed by biotinylated anti-human IgG (Vector) 5 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A